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PlasmidCloning vectorExpression vectorPhagemidCosmidSingle-strand dna-binding proteinLacuv5Circular chromosomeMolecular cloningRepliconCrisprT7 expression systemRecombinant dnaDna vaccineSelectable markerBacterial recombinationGene gunCdna libraryLibraryTransformasomeAlkaline lysisBacterial transcriptionReporter geneTransfer dnaTransfectantTransductionTransformationGene splicingTransgeneCloningConstructArtificial gene synthesisProphageTopo cloningStuffer fragmentEpisomeMicrobiological cultureSynthetic genomicsIteronChromosomeMutagenesisHorizontal gene transferPhi x 174TransformantLysogenic cyclePbr322BacteriophageExpression cassetteGenetic engineeringBacillus amyloliquefaciensOperonChloroplast dnaSomatic cell nuclear transferGenetically modified organismPlant tissue culturePolylinkerTransfectionSynthetic biologyDna virusPlasmid preparationCas12aPolymerase chain reactionTransductantAmpliconMutationTransconjugantAttbLentiviral vector in gene therapyGenomic dnaSubcloneCrispr interferenceDna shufflingGene knock-inReproductive cloningFusion proteinRapid amplification of cdna endsRetronFunctional genomicsBiobrickSexductionProkaryotic dna replicationPhage typingInsertion sequenceTranscription factorBacteriologyTi plasmidSite-directed mutagenesisMultiple cloning siteInsertional mutagenesisBacterial conjugationGenome assemblyGene therapySanger sequencingPhage displayBioprocessLoxpAmplificationRetrovirusReporterLambda phage